Cellosaurus cell line Flp-In TRex–DLD-1, TIR1-9Myc (CVCL

Cellosaurus Flp-In TRex–DLD-1, TIR1-9Myc (CVCL_F4C1)

[Text][XML][JSON]

Cell line name

Flp-In TRex–DLD-1, TIR1-9Myc

Accession

CVCL_F4C1

Resource Identification Initiative

To cite this cell line use: Flp-In TRex–DLD-1, TIR1-9Myc (RRID:CVCL_F4C1)

Comments

Population: Caucasian.
Characteristics: Expresses the TIR1 F-box protein and contains a single stably integrated FRT site for the establishment of isogenic cell lines expressing AID-tagged transgenes.
Characteristics: Used for auxin-inducible degradation of AID-tagged proteins.
Genetic integration: Method=Transduction; Gene=UniProtKB; Q7XVM8; Oryza sativa subsp. japonica TIR1-like protein Os04g0395600 (Note=With a 9xMyc tag).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Cell type: Epithelial cell of colon; CL=CL_0011108.

Disease

Colon adenocarcinoma (NCIt: C4349)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_F4C0 (Flp-In TRex–DLD-1)
Children:

CVCL_F4BY (DLD-1 T-REX Flp-ln H2BmRFP TIR1-9MyC)

Sex of cell

Male

Age at sampling

67Y