ID   Flp-In TRex–DLD-1, TIR1-9Myc
AC   CVCL_F4C1
RX   PubMed=23150568;
CC   Population: Caucasian.
CC   Characteristics: Expresses the TIR1 F-box protein and contains a single stably integrated FRT site for the establishment of isogenic cell lines expressing AID-tagged transgenes.
CC   Characteristics: Used for auxin-inducible degradation of AID-tagged proteins.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; Q7XVM8; Oryza sativa subsp. japonica TIR1-like protein Os04g0395600 (Note=With a 9xMyc tag).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Derived from site: In situ; Colon; UBERON=UBERON_0001155.
CC   Cell type: Epithelial cell of colon; CL=CL_0011108.
DI   NCIt; C4349; Colon adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_F4C0 ! Flp-In TRex–DLD-1
SX   Male
AG   67Y
CA   Cancer cell line
DT   Created: 25-06-26; Last updated: 25-06-26; Version: 1
CH   CVCL_F4BY ! DLD-1 T-REX Flp-ln H2BmRFP TIR1-9MyC
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RX   PubMed=23150568; DOI=10.1073/pnas.1216880109; PMCID=PMC3523849;
RA   Holland, Andrew J.
RA   Fachinetti, Daniele
RA   Han, Joo Seok
RA   Cleveland, Don W.
RT   "Inducible, reversible system for the rapid and complete degradation
RT   of proteins in mammalian cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:E3350-E3357(2012).
//