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Cellosaurus TRIP13 (AID) DLD-1 T-Rex Flp-In H2BmRFP+TIR1-9Myc+ (CVCL_F4BX)

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Cell line name TRIP13 (AID) DLD-1 T-Rex Flp-In H2BmRFP+TIR1-9Myc+
Synonyms DLD-1 TRIP13 (AID); TRIP13AID
Accession CVCL_F4BX
Resource Identification Initiative To cite this cell line use: TRIP13 (AID) DLD-1 T-Rex Flp-In H2BmRFP+TIR1-9Myc+ (RRID:CVCL_F4BX)
Comments Population: Caucasian.
Characteristics: Contains an inducible system enabling rapid depletion of TRIP13 via auxin-inducible degron (AID) tagging of both endogenous TRIP13 alleles.
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:12307; TRIP13 (Note=With N-ter AID and GFP tags).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P93830; Arabidopsis thaliana IAA17 (Note=Acts as an auxin-inducible degron (AID)).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q7XVM8; Oryza sativa subsp. japonica TIR1-like protein Os04g0395600 (Note=With a 9xMyc tag).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; 5YCFA; mRFP1 (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:4761; H2BC11.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Cell type: Epithelial cell of colon; CL=CL_0011108.
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_F4BY (DLD-1 T-REX Flp-ln H2BmRFP TIR1-9MyC)
Sex of cell Male
Age at sampling 67Y
Category Cancer cell line
Publications

PubMed=30341343; DOI=10.1038/s41467-018-06774-1; PMCID=PMC6195577
Dong Hyun Kim, Joo Seok Han, Peter Ly, Qiaozhen Ye, Moira A. McMahon, Kyungjae Myung, Kevin D. Corbett, Don W. Cleveland;
TRIP13 and APC15 drive mitotic exit by turnover of interphase- and unattached kinetochore-produced MCC.
Nat. Commun. 9:4354-4354(2018)

Entry history
Entry creation25-Jun-2026
Last entry update25-Jun-2026
Version number1