ID   TRIP13 (AID) DLD-1 T-Rex Flp-In H2BmRFP+TIR1-9Myc+
AC   CVCL_F4BX
SY   DLD-1 TRIP13 (AID); TRIP13AID
RX   PubMed=30341343;
CC   Population: Caucasian.
CC   Characteristics: Contains an inducible system enabling rapid depletion of TRIP13 via auxin-inducible degron (AID) tagging of both endogenous TRIP13 alleles.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:12307; TRIP13 (Note=With N-ter AID and GFP tags).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P93830; Arabidopsis thaliana IAA17 (Note=Acts as an auxin-inducible degron (AID)).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; Q7XVM8; Oryza sativa subsp. japonica TIR1-like protein Os04g0395600 (Note=With a 9xMyc tag).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; 5YCFA; mRFP1 (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:4761; H2BC11.
CC   Derived from site: In situ; Colon; UBERON=UBERON_0001155.
CC   Cell type: Epithelial cell of colon; CL=CL_0011108.
DI   NCIt; C4349; Colon adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_F4BY ! DLD-1 T-REX Flp-ln H2BmRFP TIR1-9MyC
SX   Male
AG   67Y
CA   Cancer cell line
DT   Created: 25-06-26; Last updated: 25-06-26; Version: 1
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RX   PubMed=30341343; DOI=10.1038/s41467-018-06774-1; PMCID=PMC6195577;
RA   Kim, Dong Hyun
RA   Han, Joo Seok
RA   Ly, Peter
RA   Ye, Qiaozhen
RA   McMahon, Moira A.
RA   Myung, Kyungjae
RA   Corbett, Kevin D.
RA   Cleveland, Don W.
RT   "TRIP13 and APC15 drive mitotic exit by turnover of interphase- and
RT   unattached kinetochore-produced MCC.";
RL   Nat. Commun. 9:4354-4354(2018).
//