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Cellosaurus H4-tandem fluorescent LC3B (CVCL_F1ZM)

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Cell line name H4-tandem fluorescent LC3B
Synonyms H4-tfLC3B; GFP-mCherry-LC3B H4
Accession CVCL_F1ZM
Resource Identification Initiative To cite this cell line use: H4-tandem fluorescent LC3B (RRID:CVCL_F1ZM)
Comments Population: Caucasian.
Characteristics: Using CRISPR/Cas9 tandem GFP and mCherry were inseted at the N-terminus of endogenous MAP1LC3B (LC3B). Allows the use of the GFP:mCherry ratio to monitor the delivery of GFP-mCherry-LC3B from neutral autophagosomes to acidic autolysosomes (PubMed=31692446).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Derived from site: In situ; Brain; UBERON=UBERON_0000955.
Sequence variations
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Astrocytoma (NCIt: C60781)
Astrocytoma (ORDO: Orphanet_94)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1239 (H4)
Sex of cell Male
Age at sampling 37Y
Category Transformed cell line
Publications

PubMed=31692446; DOI=10.7554/eLife.50034; PMCID=PMC6863627
Rui Jia, Juan S. Bonifacino;
Negative regulation of autophagy by UBA6-BIRC6-mediated ubiquitination of LC3.
eLife 8:e50034-e50034(2019)

Entry history
Entry creation31-Mar-2026
Last entry update31-Mar-2026
Version number1