ID   H4-tandem fluorescent LC3B
AC   CVCL_F1ZM
SY   H4-tfLC3B; GFP-mCherry-LC3B H4
RX   PubMed=31692446;
CC   Population: Caucasian.
CC   Characteristics: Using CRISPR/Cas9 tandem GFP and mCherry were inseted at the N-terminus of endogenous MAP1LC3B (LC3B). Allows the use of the GFP:mCherry ratio to monitor the delivery of GFP-mCherry-LC3B from neutral autophagosomes to acidic autolysosomes (PubMed=31692446).
CC   Sequence variation: Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Derived from site: In situ; Brain; UBERON=UBERON_0000955.
DI   NCIt; C60781; Astrocytoma
DI   ORDO; Orphanet_94; Astrocytoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_1239 ! H4
SX   Male
AG   37Y
CA   Transformed cell line
DT   Created: 31-03-26; Last updated: 31-03-26; Version: 1
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RX   PubMed=31692446; DOI=10.7554/eLife.50034; PMCID=PMC6863627;
RA   Jia, Rui
RA   Bonifacino, Juan S.
RT   "Negative regulation of autophagy by UBA6-BIRC6-mediated
RT   ubiquitination of LC3.";
RL   eLife 8:e50034-e50034(2019).
//