Cellosaurus cell line HyCyte U-87MG KO-hCD274 (CVCL

Cross-references
Cell line name	HyCyte U-87MG KO-hCD274
Synonyms	hCD274 knockout U-87MG
Accession	CVCL_F1UV
Resource Identification Initiative	To cite this cell line use: HyCyte U-87MG KO-hCD274 (RRID:CVCL_F1UV)
Comments	Population: Caucasian. Doubling time: 72-96 hours (Hysigen=CGKO-M2959). Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:17635; CD274.
Sequence variations	Mutation; HGNC; HGNC:7765; NF1; Simple; p.Phe1247Ilefs*18 (c.3739_3742delTTTG) (3737_3740delTGTT); ClinVar=VCV000420078; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:9588; PTEN; Simple; c.209+1G>T; ClinVar=VCV000229705; Zygosity=Homozygous; Note=Splice donor mutation (from parent cell line). Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease	Glioblastoma (NCIt: C3058) Glioblastoma (ORDO: Orphanet_360)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0022 (U-87MG ATCC)
Sex of cell	Male
Age at sampling	Age unspecified
Category	Cancer cell line
Cell line collections (Providers)	Hysigen; CGKO-M2959
Entry history
Entry creation	27-Nov-2025
Last entry update	27-Nov-2025
Version number	1