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Cellosaurus L-PdL-BGH4-13 (CVCL_F0TW)

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Cell line name L-PdL-BGH4-13
Synonyms L-Pdlambda-BGH4-13; L-BGH-4-13
Accession CVCL_F0TW
Resource Identification Initiative To cite this cell line use: L-PdL-BGH4-13 (RRID:CVCL_F0TW)
Comments Group: Patented cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-8536.
Characteristics: Secrete bovine GH1 at a rate of 75 muG per 5 x 10^6 cells per 24 hours. Co-transfected with pBGH-4 plasmid that contains bovine GH1 under the control of the RSV LTR and pDlambdaAT-3 that contains hamster Aprt and HSV-1 TK (PubMed=2982557).
Selected for resistance to: ChEBI; CHEBI_472552; 5-bromo-2'-deoxyuridine (Bromodeoxyuridine; BrdU; BUdR).
Selected for resistance to: ChEBI; CHEBI_77751; 8-azaadenine.
Genetic integration: Method=Transfection; Gene=UniProtKB; P01246; Bovine GH1.
Genetic integration: Method=Transfection; Gene=UniProtKB; P47952; Cricetulus griseus Aprt.
Genetic integration: Method=Transfection; Gene=UniProtKB; Q9QNF7; Human herpesvirus 1 TK.
Derived from site: In situ; Connective tissue; UBERON=UBERON_0002384.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C3H/An.
Hierarchy Parent: CVCL_0P16 (L-M(TK-,APRT-))
Sex of cell Male
Age at sampling 100D
Category Spontaneously immortalized cell line
Publications

PubMed=2982557; DOI=10.1089/dna.1985.4.23
John Joseph Kopchick, Richard Harold Malavarca, Thomas J. Livelli, Frederick Chi-Ching Leung;
Use of avian retroviral-bovine growth hormone DNA recombinants to direct expression of biologically active growth hormone by cultured fibroblasts.
DNA 4:23-31(1985)

Patent=US4686098
John Joseph Kopchick, Frederick Chi-Ching Leung, Thomas J. Livelli, Richard Harold Malavarca;
Encapsulated mouse cells transformed with avian retrovirus-bovine growth hormone DNA, and a method of administering BGH in vivo.
Patent number US4686098, 11-Aug-1987

Entry history
Entry creation27-Nov-2025
Last entry update27-Nov-2025
Version number1