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Cellosaurus H9 AAVS1-TRE3G-NGN2 TMEM192-3xHA (heterozygous) ASAH1-/- (CVCL_F0PK)

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Cell line name H9 AAVS1-TRE3G-NGN2 TMEM192-3xHA (heterozygous) ASAH1-/-
Synonyms H9 AAVS1-TRE3G-NGN2 TMEM192-3xHA::ASAH1-/-
Accession CVCL_F0PK
Resource Identification Initiative To cite this cell line use: H9 AAVS1-TRE3G-NGN2 TMEM192-3xHA (heterozygous) ASAH1-/- (RRID:CVCL_F0PK)
Comments Characteristics: Using CRISPR/Cas9 a TRE3G promoter driven NEUROG2 construct was introduced in the AAVS1 safe harbor locus (from parent cell line).
Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on one allele with a 3xHA epitope (from parent cell line).
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:735; ASAH1.
Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:13805; NEUROG2.
Miscellaneous: Cell line information from personal communication of Harper, Jeffrey Wade.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_E3KT (H9 AAVS1-TRE3G-NGN2 TMEM192-3xHA (heterozygous))
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Entry history
Entry creation14-Aug-2025
Last entry update14-Aug-2025
Version number1