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Cellosaurus HT29-MTX resistant (100 microMolar) (CVCL_F0KP)

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Cell line name HT29-MTX resistant (100 microMolar)
Synonyms HT29-MTX resistant (10-4M)
Accession CVCL_F0KP
Resource Identification Initiative To cite this cell line use: HT29-MTX resistant (100 microMolar) (RRID:CVCL_F0KP)
Comments Population: Caucasian.
Characteristics: Resistant to 100 muM of methotrexate (ECACC=15121710).
Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX).
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; HGNC:583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0320 (HT-29)
Sex of cell Female
Age at sampling 44Y
Category Cancer cell line
STR profile Source(s): ECACC=15121710

Markers:
AmelogeninX
CSF1PO11,12
D3S135815,17
D5S81811,12
D7S82010
D8S117910
D13S31711
D16S53911,12
D18S5113
D21S1129
FGA20,22
Penta D11
Penta E14,16
TH016,9
TPOX8,9
vWA17

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Cross-references
Cell line collections (Providers) ECACC; 15121710
Entry history
Entry creation14-Aug-2025
Last entry update14-Aug-2025
Version number1