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Cellosaurus U-2 OS H2B-GFP+mCherry-TRF1 (CVCL_F0KK)

[Text version]
Cell line name U-2 OS H2B-GFP+mCherry-TRF1
Accession CVCL_F0KK
Resource Identification Initiative To cite this cell line use: U-2 OS H2B-GFP+mCherry-TRF1 (RRID:CVCL_F0KK)
Comments Population: Caucasian.
Characteristics: Transduced with both a pWZL-H2B-eGFP-HygR construct and a pLPC-mCherry-TRF1-Puro construct (PubMed=38213617).
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:4761; H2BC11.
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11728; TERF1.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=38213617; DOI=10.1016/j.isci.2023.108655; PMCID=PMC10783591
Robert Lu, Christopher B. Nelson, Samuel Rogers, Anthony J. Cesare, Alexander Peter Sobinoff, Hilda A. Pickett;
Distinct modes of telomere synthesis and extension contribute to alternative lengthening of telomeres.
iScience 27:108655.1-10865.21(2024)

Entry history
Entry creation14-Aug-2025
Last entry update14-Aug-2025
Version number1