ID   U-2 OS H2B-GFP+mCherry-TRF1
AC   CVCL_F0KK
RX   PubMed=38213617;
CC   Population: Caucasian.
CC   Characteristics: Transduced with both a pWZL-H2B-eGFP-HygR construct and a pLPC-mCherry-TRF1-Puro construct (PubMed=38213617).
CC   Sequence variation: Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:4761; H2BC11.
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11728; TERF1.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0042 ! U2OS
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 14-08-25; Last updated: 14-08-25; Version: 1
//
RX   PubMed=38213617; DOI=10.1016/j.isci.2023.108655; PMCID=PMC10783591;
RA   Lu, Robert
RA   Nelson, Christopher B.
RA   Rogers, Samuel
RA   Cesare, Anthony J.
RA   Sobinoff, Alexander Peter
RA   Pickett, Hilda A.
RT   "Distinct modes of telomere synthesis and extension contribute to
RT   alternative lengthening of telomeres.";
RL   iScience 27:108655.1-10865.21(2024).
//