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Cellosaurus KUST_CyESCcyc_WT1tdt (CVCL_E9XX)

[Text version]
Cell line name KUST_CyESCcyc_WT1tdt
Synonyms WT1-205; cyc-WT1-tdt; WT1-Ki-cyc; WT1-2A-tdTomato cyESC
Accession CVCL_E9XX
Resource Identification Initiative To cite this cell line use: KUST_CyESCcyc_WT1tdt (RRID:CVCL_E9XX)
Comments Group: Non-human primate cell line.
Characteristics: Using CRISPR/Cas9 as T2A-tdTomato construct was introduced at the C-terminus of the WT1 gene (PubMed=40466381).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Caution: The parent ESC cell line is not indicated in the publication.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) (NCBI Taxonomy: 9541)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=40466381; DOI=10.1016/j.scr.2025.103742
Ahjol Hyraht, Feng-Lin Zhan, Rong-Rong Guo;
Generation of WT1-tdTomato knock-in cynomolgus monkey embryonic stem cell line, WT1-205 using CRISPR/CAS9-based gene targeting.
Stem Cell Res. 87:103742-103742(2025)

Entry history
Entry creation14-Aug-2025
Last entry update14-Aug-2025
Version number1