ID   KUST_CyESCcyc_WT1tdt
AC   CVCL_E9XX
SY   WT1-205; cyc-WT1-tdt; WT1-Ki-cyc; WT1-2A-tdTomato cyESC
RX   PubMed=40466381;
CC   Group: Non-human primate cell line.
CC   Characteristics: Using CRISPR/Cas9 as T2A-tdTomato construct was introduced at the C-terminus of the WT1 gene (PubMed=40466381).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
CC   Caution: The parent ESC cell line is not indicated in the publication.
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9541; ! Macaca fascicularis (Crab-eating macaque)
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 14-08-25; Last updated: 14-08-25; Version: 1
//
RX   PubMed=40466381; DOI=10.1016/j.scr.2025.103742;
RA   Hyraht, Ahjol
RA   Zhan, Feng-Lin
RA   Guo, Rong-Rong
RT   "Generation of WT1-tdTomato knock-in cynomolgus monkey embryonic stem
RT   cell line, WT1-205 using CRISPR/CAS9-based gene targeting.";
RL   Stem Cell Res. 87:103742-103742(2025).
//