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Cellosaurus U251-hrGFP (CVCL_E9DU)

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Cell line name U251-hrGFP
Synonyms U251-GFP
Accession CVCL_E9DU
Resource Identification Initiative To cite this cell line use: U251-hrGFP (RRID:CVCL_E9DU)
Comments Population: Caucasian.
Characteristics: Using CRISPR/Cas9 a CAG-hrGFP-PuroR construct was introduced in the AAVS1 safe harbor locus (PubMed=32591581).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 84CGO; rrGFP (Note=hrGFP; luorescent protein published in 2007, green fluorescent protein derived from R.reniformis).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Brain, parietal lobe; UBERON=UBERON_0001872.
Sequence variations
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Glu242Valfs*15 (c.723_724dupTG); ClinVar=VCV000536553; Zygosity=Unspecified (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Unspecified (from parent cell line).
Disease Astrocytoma (NCIt: C60781)
Astrocytoma (ORDO: Orphanet_94)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0021 (U-251MG)
Sex of cell Male
Age at sampling 75Y
Category Cancer cell line
Publications

PubMed=32591581; DOI=10.1038/s41598-020-67492-7; PMCID=PMC7320147
Tamilarasan K. Palaniappan, Lina Slekiene, Anna-Karin Jonasson, Jonathan D. Gilthorpe, Lena Gunhaga;
CAM-Delam: an in vivo approach to visualize and quantify the delamination and invasion capacity of human cancer cells.
Sci. Rep. 10:10472.1-10472.11(2020)

Entry history
Entry creation14-Aug-2025
Last entry update14-Aug-2025
Version number1