ID   U251-hrGFP
AC   CVCL_E9DU
SY   U251-GFP
RX   PubMed=32591581;
CC   Population: Caucasian.
CC   Characteristics: Using CRISPR/Cas9 a CAG-hrGFP-PuroR construct was introduced in the AAVS1 safe harbor locus (PubMed=32591581).
CC   Sequence variation: Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Glu242Valfs*15 (c.723_724dupTG); ClinVar=VCV000536553; Zygosity=Unspecified (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Unspecified (from parent cell line).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 84CGO; rrGFP (Note=hrGFP; luorescent protein published in 2007, green fluorescent protein derived from R.reniformis).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Derived from site: In situ; Brain, parietal lobe; UBERON=UBERON_0001872.
DI   NCIt; C60781; Astrocytoma
DI   ORDO; Orphanet_94; Astrocytoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0021 ! U-251MG
SX   Male
AG   75Y
CA   Cancer cell line
DT   Created: 14-08-25; Last updated: 14-08-25; Version: 1
//
RX   PubMed=32591581; DOI=10.1038/s41598-020-67492-7; PMCID=PMC7320147;
RA   Palaniappan, Tamilarasan K.
RA   Slekiene, Lina
RA   Jonasson, Anna-Karin
RA   Gilthorpe, Jonathan D.
RA   Gunhaga, Lena
RT   "CAM-Delam: an in vivo approach to visualize and quantify the
RT   delamination and invasion capacity of human cancer cells.";
RL   Sci. Rep. 10:10472.1-10472.11(2020).
//