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Cellosaurus A549-hrGFP (CVCL_E9DR)

[Text version]
Cell line name A549-hrGFP
Synonyms A549-GFP
Accession CVCL_E9DR
Resource Identification Initiative To cite this cell line use: A549-hrGFP (RRID:CVCL_E9DR)
Comments Population: Caucasian.
Characteristics: Using CRISPR/Cas9 a CAG-hrGFP-PuroR construct was introduced in the AAVS1 safe harbor locus (PubMed=32591581).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 84CGO; rrGFP (Note=hrGFP; luorescent protein published in 2007, green fluorescent protein derived from R.reniformis).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0023 (A-549)
Sex of cell Male
Age at sampling 58Y
Category Cancer cell line
Publications

PubMed=32591581; DOI=10.1038/s41598-020-67492-7; PMCID=PMC7320147
Tamilarasan K. Palaniappan, Lina Slekiene, Anna-Karin Jonasson, Jonathan D. Gilthorpe, Lena Gunhaga;
CAM-Delam: an in vivo approach to visualize and quantify the delamination and invasion capacity of human cancer cells.
Sci. Rep. 10:10472.1-10472.11(2020)

Entry history
Entry creation14-Aug-2025
Last entry update14-Aug-2025
Version number1