ID   A549-hrGFP
AC   CVCL_E9DR
SY   A549-GFP
RX   PubMed=32591581;
CC   Population: Caucasian.
CC   Characteristics: Using CRISPR/Cas9 a CAG-hrGFP-PuroR construct was introduced in the AAVS1 safe harbor locus (PubMed=32591581).
CC   Sequence variation: Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 84CGO; rrGFP (Note=hrGFP; luorescent protein published in 2007, green fluorescent protein derived from R.reniformis).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Derived from site: In situ; Lung; UBERON=UBERON_0002048.
DI   NCIt; C3512; Lung adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0023 ! A-549
SX   Male
AG   58Y
CA   Cancer cell line
DT   Created: 14-08-25; Last updated: 14-08-25; Version: 1
//
RX   PubMed=32591581; DOI=10.1038/s41598-020-67492-7; PMCID=PMC7320147;
RA   Palaniappan, Tamilarasan K.
RA   Slekiene, Lina
RA   Jonasson, Anna-Karin
RA   Gilthorpe, Jonathan D.
RA   Gunhaga, Lena
RT   "CAM-Delam: an in vivo approach to visualize and quantify the
RT   delamination and invasion capacity of human cancer cells.";
RL   Sci. Rep. 10:10472.1-10472.11(2020).
//