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Cellosaurus 5555-EKAREV-NLS (CVCL_E9CK)

[Text version]
Cell line name 5555-EKAREV-NLS
Accession CVCL_E9CK
Resource Identification Initiative To cite this cell line use: 5555-EKAREV-NLS (RRID:CVCL_E9CK)
Comments Characteristics: Carries the FRET biosensor EKARrEV-NLS, which consists of a donor fluorescent protein CFP, an ERK substrate peptide derived from Cdc25, an optimized linker, a FHA1 phosphate binding domain, an acceptor fluorescent protein YFP and a nuclear localization signal (PubMed=25873177).
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; ZRLU4; CFP (Note=Cyan fluorescent protein derived from GFP).
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP).
Genetic integration: Method=Transgenic mouse; Gene=MGI; MGI:1352467; Esr1 (Note=Ligand binding domain; with p.Gly525Arg. Fused to Cre = CreERT2).
Breed/subspecies: C57BL/6 BRAF+/LSL-BRAFV600E;Tyr::CreERT2+/o transgenic.
Sequence variations
  • Mutation; MGI; MGI:88190; Braf; Simple; p.Val637Glu (c.1910T>A); Zygosity=Heterozygous (PubMed=25873177).
Disease Mouse melanoma (NCIt: C21790)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Hierarchy Parent: CVCL_E9CJ (5555)
Sex of cell Sex unspecified
Age at sampling Age unspecified
Category Cancer cell line
Publications

PubMed=25873177; DOI=10.1016/j.ccell.2015.03.008; PMCID=PMC4402404
Eishu Hirata, Maria Romina Girotti, Amaya Viros, Steven Hooper, Bradley Spencer-Dene, Michiyuki Matsuda, James Larkin, Richard Marais, Erik Sahai;
Intravital imaging reveals how BRAF inhibition generates drug-tolerant microenvironments with high integrin beta1/FAK signaling.
Cancer Cell 27:574-588(2015)

PubMed=26414886; DOI=10.1111/pcmr.12424; PMCID=PMC4737278
Heike Seifert, Eishu Hirata, Martin Eric Gore, Komel Khabra, Christina Messiou, James Larkin, Erik Sahai;
Extrinsic factors can mediate resistance to BRAF inhibition in central nervous system melanoma metastases.
Pigment Cell Melanoma Res. 29:92-100(2016)

Entry history
Entry creation14-Aug-2025
Last entry update14-Aug-2025
Version number1