Cellosaurus WIZ02e-H9CAGhM4Di (CVCL_E9C4)
| Cell line name | WIZ02e-H9CAGhM4Di |
|---|---|
| Accession | CVCL_E9C4 |
| Resource Identification Initiative | To cite this cell line use: WIZ02e-H9CAGhM4Di (RRID:CVCL_E9C4) |
| Comments | Registration: Swiss research registry; BAG-hES-IMP-0104. Characteristics: Expresses hM4Di, a modified form of human CHRM4 that can no longer bind the native ligand, acetylcholine, but is activated by the inert clozapine metabolite clozapine-N-oxide (CNO), engaging the Gi signaling pathway (PubMed=27133795). Characteristics: Using CRISPR/Cas9 a T2A-PuroR-CAG-hM4Di-mCherry construct was introduced in the AAVS1 safe harbor locus (PubMed=27133795). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)). Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:1953; CHRM4 (Note=With p.Tyr113Cys and p.Ala203Gly = hM4Di). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_9773 (WA09) |
| Sex of cell | Female |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=27133795; DOI=10.1016/j.stem.2016.03.014; PMCID=PMC4892985 |
| Cross-references | |
| Cell line collections (Providers) | WiCell; wiz02e-h9caghm4di |
| Entry history | |
| Entry creation | 14-Aug-2025 |
| Last entry update | 14-Aug-2025 |
| Version number | 1 |