Cellosaurus S2R+-PE2-P2A-EGFP (CVCL_E8FV)
| Cell line name | S2R+-PE2-P2A-EGFP |
|---|---|
| Accession | CVCL_E8FV |
| Resource Identification Initiative | To cite this cell line use: S2R+-PE2-P2A-EGFP (RRID:CVCL_E8FV) |
| Comments | Group: Insect cell line. Characteristics: Transfected with a PE2-P2A-EGFP construct containing the prime editor protein (PE2) which is a fusion between the S.pyogenes Cas9(H840A) and an engineered Moloney murine leukemia virus (MMLV) reverse transcriptase domain. PE2 enables gene editing at potentially any locus in the genome by transfecting a pegRNA specific for the desired target locus (DGRC=349). Genetic integration: Method=Protein trap; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)). Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.His840Ala). Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922. |
| Species of origin | Drosophila melanogaster (Fruit fly)
(NCBI Taxonomy: 7227)
Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276. |
| Hierarchy | Parent: CVCL_0Q79 (S2R+-NPT005) |
| Sex of cell | Male |
| Age at sampling | Late embryonic stage |
| Category | Spontaneously immortalized cell line |
| Cross-references | |
| Cell line collections (Providers) | DGRC; 349 |
| Cell line databases/resources | FlyBase_Cell_line; FBtc0000349 |
| Entry history | |
| Entry creation | 14-Aug-2025 |
| Last entry update | 14-Aug-2025 |
| Version number | 1 |