ID   S2R+-PE2-P2A-EGFP
AC   CVCL_E8FV
DR   DGRC; 349
DR   FlyBase_Cell_line; FBtc0000349
CC   Group: Insect cell line.
CC   Characteristics: Transfected with a PE2-P2A-EGFP construct containing the prime editor protein (PE2) which is a fusion between the S.pyogenes Cas9(H840A) and an engineered Moloney murine leukemia virus (MMLV) reverse transcriptase domain. PE2 enables gene editing at potentially any locus in the genome by transfecting a pegRNA specific for the desired target locus (DGRC=349).
CC   Genetic integration: Method=Protein trap; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.His840Ala).
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276.
OX   NCBI_TaxID=7227; ! Drosophila melanogaster (Fruit fly)
HI   CVCL_0Q79 ! S2R+-NPT005
SX   Male
AG   Late embryonic stage
CA   Spontaneously immortalized cell line
DT   Created: 14-08-25; Last updated: 14-08-25; Version: 1
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