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Cellosaurus HCC827(gFGFR1) (CVCL_E8FJ)

[Text version]
Cell line name HCC827(gFGFR1)
Accession CVCL_E8FJ
Resource Identification Initiative To cite this cell line use: HCC827(gFGFR1) (RRID:CVCL_E8FJ)
Comments Population: Caucasian.
Characteristics: FGFR1 gene expression was genetically up-regulated. First transduced with a CRISPR-based Synergistic Activation Mediator (SAM) system composed of two lentiviral vectors dCAS-VP64_Blast (EF1a-dCas9-VP64-Bsd-WPE) and MS2-P65-HSF1-Hygro (EF1a-MS2(N55K)-SV40(NLS)-RELA-HSF1-hygR-WPRE) and then a PiggyBac transposon containing three unique SAM compatible gRNAs spanning the promoter region of FGFR1 (gFGFR1) and a puroR-IRES-AsRed-CMV construct (PubMed=30562682).
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; 8TL1P; AsRed2 (Note=Red fluorescent protein derived from Anemonia sulcata).
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:5224; HSF1.
Genetic integration: Method=Transduction; Gene=MGI; MGI:103290; Rela (Note=With p.Met1_Leu368del).
Genetic integration: Method=Transduction; Gene=UniProtKB; P03612; Escherichia phage MS2 capsid protein (Note=With p.Asn56Lys).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
Omics: Transcriptomics; Microarray.
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Mutation; HGNC; HGNC:3236; EGFR; Simple; p.Glu746_Ala750del (c.2236_2250del15); ClinVar=VCV000177620; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Val218del (c.646_648GTG[2]) (c.652_654delGTG); ClinVar=VCV000634761; Zygosity=Homozygous (from parent cell line).
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_2063 (HCC827)
Sex of cell Female
Age at sampling 39Y
Category Cancer cell line
Publications

PubMed=30562682; DOI=10.1016/j.tranon.2018.11.017; PMCID=PMC6297127
Gammelgaard K.R., Vad-Nielsen J., Clement M.S., Weiss S., Daugaard T.F., Dagnaes-Hansen F., Meldgaard P., Sorensen B.S., Nielsen A.L.
Up-regulated FGFR1 expression as a mediator of intrinsic TKI resistance in EGFR-mutated NSCLC.
Transl. Oncol. 12:432-440(2019)

Cross-references
Cell line collections (Providers) ABM; T6464
Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1