ID   HCC827(gFGFR1)
AC   CVCL_E8FJ
DR   ABM; T6464
RX   PubMed=30562682;
CC   Population: Caucasian.
CC   Characteristics: FGFR1 gene expression was genetically up-regulated. First transduced with a CRISPR-based Synergistic Activation Mediator (SAM) system composed of two lentiviral vectors dCAS-VP64_Blast (EF1a-dCas9-VP64-Bsd-WPE) and MS2-P65-HSF1-Hygro (EF1a-MS2(N55K)-SV40(NLS)-RELA-HSF1-hygR-WPRE) and then a PiggyBac transposon containing three unique SAM compatible gRNAs spanning the promoter region of FGFR1 (gFGFR1) and a puroR-IRES-AsRed-CMV construct (PubMed=30562682).
CC   Sequence variation: Mutation; HGNC; HGNC:3236; EGFR; Simple; p.Glu746_Ala750del (c.2236_2250del15); ClinVar=VCV000177620; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Val218del (c.646_648GTG[2]) (c.652_654delGTG); ClinVar=VCV000634761; Zygosity=Homozygous (from parent cell line).
CC   Genetic integration: Method=PiggyBac transposition; Gene=FPbase; 8TL1P; AsRed2 (Note=Red fluorescent protein derived from Anemonia sulcata).
CC   Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:5224; HSF1.
CC   Genetic integration: Method=Transduction; Gene=MGI; MGI:103290; Rela (Note=With p.Met1_Leu368del).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P03612; Escherichia phage MS2 capsid protein (Note=With p.Asn56Lys).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Omics: Transcriptomics; Microarray.
CC   Derived from site: In situ; Lung; UBERON=UBERON_0002048.
DI   NCIt; C3512; Lung adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_2063 ! HCC827
SX   Female
AG   39Y
CA   Cancer cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
//
RX   PubMed=30562682; DOI=10.1016/j.tranon.2018.11.017; PMCID=PMC6297127;
RA   Gammelgaard K.R., Vad-Nielsen J., Clement M.S., Weiss S., Daugaard T.F.,
RA   Dagnaes-Hansen F., Meldgaard P., Sorensen B.S., Nielsen A.L.;
RT   "Up-regulated FGFR1 expression as a mediator of intrinsic TKI
RT   resistance in EGFR-mutated NSCLC.";
RL   Transl. Oncol. 12:432-440(2019).
//