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Cellosaurus MAGIC-5 (CVCL_E8AM)

[Text version]
Cell line name MAGIC-5
Synonyms MAGIC-5A 1-10; HeLa/CD4+ cell clone 1-10
Accession CVCL_E8AM
Resource Identification Initiative To cite this cell line use: MAGIC-5 (RRID:CVCL_E8AM)
Comments Population: African American.
Characteristics: Transfected with a construct pJK2 containing the HIV-1 LTR fused to a lacZ reporter gene that contains a MPKKKRK N-terminal sequence directing it to the nucleus (from parent cell line).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1678; CD4.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:1606; CCR5.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_D259 (HeLa-MAGI)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
Publications

PubMed=11158746; DOI=10.1128/AAC.45.2.495-501.2001; PMCID=PMC90318
Hachiya A., Aizawa-Matsuoka S., Tanaka M., Takahashi Y., Ida S., Gatanaga H., Hirabayashi Y., Kojima A., Tatsumi M., Oka S.
Rapid and simple phenotypic assay for drug susceptibility of human immunodeficiency virus type 1 using CCR5-expressing HeLa/CD4(+) cell clone 1-10 (MAGIC-5).
Antimicrob. Agents Chemother. 45:495-501(2001)

Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1