Cellosaurus logo
expasy logo

Cellosaurus iLite TNF-alpha Assay Ready Cells (CVCL_E7RU)

[Text version]
Cell line name iLite TNF-alpha Assay Ready Cells
Synonyms KJL-Luc
Accession CVCL_E7RU
Resource Identification Initiative To cite this cell line use: iLite TNF-alpha Assay Ready Cells (RRID:CVCL_E7RU)
Comments Population: Caucasian.
Characteristics: Genetically engineered and optimized to respond to TNF-alpha resulting in the specific expression of firefly luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla luciferase reporter gene construct, under control of a constitutive promoter (Svar Life Science=BM3044).
Characteristics: The luciferase reporter gene is under the control of 5 tandem copies of the NF-kappaB response element and the Renilla luciferase is under the control of a constitutive minimal TK promoter (PubMed=21910993).
Genetic integration: Method=Transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla reniformis renilla-type luciferase.
Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene fusion; HGNC; HGNC:76; ABL1 + HGNC; HGNC:1014; BCR; Name(s)=BCR-ABL1, BCR-ABL; Note=BCR exon 14 fused to ABL1 exon 2 (b3a2 transcript) (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Gln136fs*13 (c.406_407insC); Zygosity=Homozygous (from parent cell line).
Disease Blast phase chronic myelogenous leukemia, BCR-ABL1 positive (NCIt: C9110)
Chronic myeloid leukemia (ORDO: Orphanet_521)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_E7RV (KK-Luc)
Sex of cell Female
Age at sampling 53Y
Category Cancer cell line
Web pages Provider; Svar Life Science; BM3044; https://www.svarlifescience.com/products/bm3044
Publications

PubMed=21910993; DOI=10.1016/j.jim.2011.08.022
Christophe Lallemand, Nadia Kavrochorianou, Casper Steenholdt, Klaus Bendtzen, Mark Andrew Ainsworth, Jean-Francois Meritet, Brigitte Blanchard, Pierre Lebon, Peter Taylor, Peter Charles, Saba Alzabin, Michael G. Tovey;
Reporter gene assay for the quantification of the activity and neutralizing antibody response to TNFalpha antagonists.
J. Immunol. Methods 373:229-239(2011)

Entry history
Entry creation10-Apr-2025
Last entry update14-Aug-2025
Version number2