Cellosaurus HeLa Luc/705 (CVCL_E7IX)
| Cell line name | HeLa Luc/705 |
|---|---|
| Accession | CVCL_E7IX |
| Resource Identification Initiative | To cite this cell line use: HeLa Luc/705 (RRID:CVCL_E7IX) |
| Comments | Population: African American. Characteristics: Transfected with a construct containing a luciferase gene interrupted by a mutated human beta-globin intron 2, HBB IVS2-705. The mutation in the intron causes aberrant splicing of luciferase pre-mRNA, preventing translation of luciferase. However, treatment of the cells with a 2'-O-methyl-oligoribonucleotide targeted to the aberrant splice sites induces correct splicing, restoring luciferase activity (PubMed=9572837). Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del). Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18). Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002. |
| Disease | Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_V352 (HeLa Tet-Off) |
| Sex of cell | Female |
| Age at sampling | 30Y6M |
| Category | Cancer cell line |
| Web pages | Provider; UNC OTC; 16-0176; https://unc.flintbox.com/technologies/2512ea18-bd35-4199-8769-0c6bd96cbcdc |
| Publications | PubMed=9572837; DOI=10.1021/bi980300h |
| Entry history | |
| Entry creation | 10-Apr-2025 |
| Last entry update | 10-Apr-2025 |
| Version number | 1 |