ID   HeLa Luc/705
AC   CVCL_E7IX
RX   PubMed=9572837;
WW   Provider; UNC OTC; 16-0176; https://unc.flintbox.com/technologies/2512ea18-bd35-4199-8769-0c6bd96cbcdc
CC   Population: African American.
CC   Characteristics: Transfected with a construct containing a luciferase gene interrupted by a mutated human beta-globin intron 2, HBB IVS2-705. The mutation in the intron causes aberrant splicing of luciferase pre-mRNA, preventing translation of luciferase. However, treatment of the cells with a 2'-O-methyl-oligoribonucleotide targeted to the aberrant splice sites induces correct splicing, restoring luciferase activity (PubMed=9572837).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC   Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
DI   NCIt; C27677; Human papillomavirus-related endocervical adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_V352 ! HeLa Tet-Off
SX   Female
AG   30Y6M
CA   Cancer cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   PubMed=9572837; DOI=10.1021/bi980300h;
RA   Kang S.-H., Cho M.-J., Kole R.;
RT   "Up-regulation of luciferase gene expression with antisense
RT   oligonucleotides: implications and applications in functional assay
RT   development.";
RL   Biochemistry 37:6235-6239(1998).
//