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Cellosaurus IPKM (CVCL_E7C1)

[Text version]
Cell line name IPKM
Synonyms Immortalized Porcine Kidney-derived Macrophage
Accession CVCL_E7C1
Resource Identification Initiative To cite this cell line use: IPKM (RRID:CVCL_E7C1)
Comments From: Institute of Agrobiological Sciences, National Agriculture and Food Research; Tsukuba; Japan.
Virology: Highly susceptible to infection by African swine fever virus (ASFV). Supports the propagation of both virulent and cell-adapted isolates of ASFV (PubMed=33637799).
Doubling time: ~4 days (PubMed=28871285).
Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transduction; Gene=VGNC; VGNC:93883; TERT.
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Cell type: Macrophage; CL=CL_0000235.
Species of origin Sus scrofa (Pig) (NCBI Taxonomy: 9823)
Sex of cell Female
Age at sampling 6D
Category Transformed cell line
Web pages Info; NARO; -; https://www.naro.go.jp/english/topics/laboratory/niah/138816.html
Publications

PubMed=28871285; DOI=10.3389/fvets.2017.00132; PMCID=PMC5566601
Takenouchi T., Kitani H., Suzuki S., Nakai M., Fuchimoto D.-i., Tsukimoto M., Shinkai H., Sato M., Uenishi H.
Immortalization and characterization of porcine macrophages that had been transduced with lentiviral vectors encoding the SV40 large T antigen and porcine telomerase reverse transcriptase.
Front. Vet. Sci. 4:132.1-132.9(2017)

PubMed=33637799; DOI=10.1038/s41598-021-84237-2; PMCID=PMC7910288
Masujin K., Kitamura T., Kameyama K.-i., Okadera K., Nishi T., Takenouchi T., Kitani H., Kokuho T.
An immortalized porcine macrophage cell line competent for the isolation of African swine fever virus.
Sci. Rep. 11:4759.1-4759.11(2021)

PubMed=40003039; DOI=10.3390/ani15040558; PMCID=PMC11851356
Haraguchi S., Takenouchi T., Masujin K., Suzuki S., Kokuho T., Uenishi H.
Development of serum-free culture systems for an immortalized porcine kidney-derived macrophage cell line.
Animals (Basel) 15:558.1-558.15(2025)

Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1