ID   IPKM
AC   CVCL_E7C1
SY   Immortalized Porcine Kidney-derived Macrophage
RX   PubMed=28871285;
RX   PubMed=33637799;
RX   PubMed=40003039;
WW   Info; NARO; -; https://www.naro.go.jp/english/topics/laboratory/niah/138816.html
CC   From: Institute of Agrobiological Sciences, National Agriculture and Food Research; Tsukuba; Japan.
CC   Virology: Highly susceptible to infection by African swine fever virus (ASFV). Supports the propagation of both virulent and cell-adapted isolates of ASFV (PubMed=33637799).
CC   Doubling time: ~4 days (PubMed=28871285).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Genetic integration: Method=Transduction; Gene=VGNC; VGNC:93883; TERT.
CC   Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
CC   Cell type: Macrophage; CL=CL_0000235.
OX   NCBI_TaxID=9823; ! Sus scrofa (Pig)
SX   Female
AG   6D
CA   Transformed cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   PubMed=28871285; DOI=10.3389/fvets.2017.00132; PMCID=PMC5566601;
RA   Takenouchi T., Kitani H., Suzuki S., Nakai M., Fuchimoto D.-i.,
RA   Tsukimoto M., Shinkai H., Sato M., Uenishi H.;
RT   "Immortalization and characterization of porcine macrophages that had
RT   been transduced with lentiviral vectors encoding the SV40 large T
RT   antigen and porcine telomerase reverse transcriptase.";
RL   Front. Vet. Sci. 4:132.1-132.9(2017).
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RX   PubMed=33637799; DOI=10.1038/s41598-021-84237-2; PMCID=PMC7910288;
RA   Masujin K., Kitamura T., Kameyama K.-i., Okadera K., Nishi T.,
RA   Takenouchi T., Kitani H., Kokuho T.;
RT   "An immortalized porcine macrophage cell line competent for the
RT   isolation of African swine fever virus.";
RL   Sci. Rep. 11:4759.1-4759.11(2021).
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RX   PubMed=40003039; DOI=10.3390/ani15040558; PMCID=PMC11851356;
RA   Haraguchi S., Takenouchi T., Masujin K., Suzuki S., Kokuho T.,
RA   Uenishi H.;
RT   "Development of serum-free culture systems for an immortalized porcine
RT   kidney-derived macrophage cell line.";
RL   Animals (Basel) 15:558.1-558.15(2025).
//