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Cellosaurus WAe001-A-2E (CVCL_E7AS)

[Text version]
Cell line name WAe001-A-2E
Synonyms H1-ASCL1-mCherry; GZLe014-A-2E
Accession CVCL_E7AS
Resource Identification Initiative To cite this cell line use: WAe001-A-2E (RRID:CVCL_E7AS)
Comments From: Guangzhou Laboratory; Guangzhou City; China.
Characteristics: Using CRISPR/Cas9 a P2A-mCherry-LoxP-PGK-Puro-LoxP construct was inserted at the C-terminus of both alleles of ASCL1 (PubMed=39059080).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9771 (WA01)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=39059080; DOI=10.1016/j.scr.2024.103500
Jiang S.-S., Dai T.-K., Li Q., Xu T., Zhang W., Sun J.-Q., Liu H.-S.
Generation of ASCL1-mCherry knock-in reporter in human embryonic stem cell line, WAe001-A-2E, using CRISPR/Cas9-based gene targeting.
Stem Cell Res. 80:103500-103500(2024)

Cross-references
Cell line databases/resources hPSCreg; WAe001-A-2E
Biological sample resources BioSamples; SAMEA115345313
Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1