ID   WAe001-A-2E
AC   CVCL_E7AS
SY   H1-ASCL1-mCherry; GZLe014-A-2E
DR   BioSamples; SAMEA115345313
DR   hPSCreg; WAe001-A-2E
RX   PubMed=39059080;
CC   From: Guangzhou Laboratory; Guangzhou City; China.
CC   Characteristics: Using CRISPR/Cas9 a P2A-mCherry-LoxP-PGK-Puro-LoxP construct was inserted at the C-terminus of both alleles of ASCL1 (PubMed=39059080).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_9771 ! WA01
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   PubMed=39059080; DOI=10.1016/j.scr.2024.103500;
RA   Jiang S.-S., Dai T.-K., Li Q., Xu T., Zhang W., Sun J.-Q., Liu H.-S.;
RT   "Generation of ASCL1-mCherry knock-in reporter in human embryonic stem
RT   cell line, WAe001-A-2E, using CRISPR/Cas9-based gene targeting.";
RL   Stem Cell Res. 80:103500-103500(2024).
//