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Cellosaurus HT29-27H (CVCL_E5ZB)

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Cell line name HT29-27H
Synonyms HT29-Cl.27H; HT-29-Cl.27H; HT29-27 clone H; HT29 line 27 clone H; Cl.27H
Accession CVCL_E5ZB
Resource Identification Initiative To cite this cell line use: HT29-27H (RRID:CVCL_E5ZB)
Comments Population: Caucasian.
Characteristics: Obtained after treatment of parent cell line in polyethylene glycol (PEG). Two types of differentiated cells are present, namely, enterocytic cells and mucus-secreting goblet cells. This clone secretes mucus (PubMed=3281752).
Doubling time: 24.8 +- 0.7 hours (PubMed=3281752).
Misspelling: HT-29-C1.27H; ABM=T8211; Note=In old versions, corrected in Jan 2025.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; HGNC:583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_E5Z7 (HT29-27)
Sex of cell Female
Age at sampling 44Y
Category Cancer cell line
Publications

PubMed=3281752
Christian L. Laboisse, Jean-Jose Maoret, Nicole Triadou, Chantal Augeron;
Restoration by polyethylene glycol of characteristics of intestinal differentiation in subpopulations of the human colonic adenocarcinoma cell line HT29.
Cancer Res. 48:2498-2504(1988)

Cross-references
Cell line collections (Providers) ABM; T8211
Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1