ID   HT29-27H
AC   CVCL_E5ZB
SY   HT29-Cl.27H; HT-29-Cl.27H; HT29-27 clone H; HT29 line 27 clone H; Cl.27H
DR   ABM; T8211
RX   PubMed=3281752;
CC   Population: Caucasian.
CC   Characteristics: Obtained after treatment of parent cell line in polyethylene glycol (PEG). Two types of differentiated cells are present, namely, enterocytic cells and mucus-secreting goblet cells. This clone secretes mucus (PubMed=3281752).
CC   Doubling time: 24.8 +- 0.7 hours (PubMed=3281752).
CC   Sequence variation: Mutation; HGNC; HGNC:583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
CC   Misspelling: HT-29-C1.27H; ABM=T8211; Note=In old versions, corrected in Jan 2025.
CC   Derived from site: In situ; Colon; UBERON=UBERON_0001155.
DI   NCIt; C4349; Colon adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_E5Z7 ! HT29-27
SX   Female
AG   44Y
CA   Cancer cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   PubMed=3281752;
RA   Laboisse C.L., Maoret J.-J., Triadou N., Augeron C.;
RT   "Restoration by polyethylene glycol of characteristics of intestinal
RT   differentiation in subpopulations of the human colonic adenocarcinoma
RT   cell line HT29.";
RL   Cancer Res. 48:2498-2504(1988).
//