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Cellosaurus CHO FIX.1F (CVCL_E4JT)

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Cell line name CHO FIX.1F
Synonyms FIX.1F
Accession CVCL_E4JT
Resource Identification Initiative To cite this cell line use: CHO FIX.1F (RRID:CVCL_E4JT)
Comments Biotechnology: Produces recombinant human factor IX sold under the brand name of BeneFIX.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:3551; F9.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:8568; FURIN (Note=Soluble version).
Genetic integration: Method=Transfection; Gene=MGI; MGI:94890; Dhfr.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Transformant: NCIt; C44386; Gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Heterozygous (from parent cell line).
  • Mutation; UniProtKB; Q2MH30; Dhfr; Simple; p.Thr137Arg (c.410G>C); Zygosity=Heterozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_1977 (CHO-DXB11)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=9565160
Scott Harrison, S. Robert Adamson, Duane Bonam, Skye Brodeur, Timothy S. Charlebois, Brian Clancy, Robert J. Costigan, Denis Drapeau, M.A. Hamilton, K. Hanley, Brian D. Kelley ...Show all 19 authors... , Andrea Knight, Mark W. Leonard, M. McCarthy, P. Oakes, Kim Sterl, Mary Switzer, Rochelle Walsh, William Barry Foster; Show fewer authors
The manufacturing process for recombinant factor IX.
Semin. Hematol. 35 Suppl. 2:4-10(1998)

DOI=10.1007/978-94-017-0926-2_3
John B. Edwards, Neil Kirby;
Recombinant coagulation factor IX (BeneFIX).
(In book chapter) Biopharmaceuticals, an industrial perspective; Walsh, Gary & Murphy, Brendan (eds.); pp.73-108; Springer; Dordrecht; Netherlands (1999)

Entry history
Entry creation19-Dec-2024
Last entry update14-Aug-2025
Version number2