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Cellosaurus MSA-5 pB-rtTA(#4)-NGN2-puro-SNAP (CVCL_E3D0)

[Text version]
Cell line name MSA-5 pB-rtTA(#4)-NGN2-puro-SNAP
Accession CVCL_E3D0
Resource Identification Initiative To cite this cell line use: MSA-5 pB-rtTA(#4)-NGN2-puro-SNAP (RRID:CVCL_E3D0)
Comments Part of: Mass General Brigham Stem Cells in Neurodegeneration [SCiN] panel.
Characteristics: Transposed with a piggyBac-rtTA(TRE4G)-NGN2-T2A-PuroR-IRES-SNAP-tag construct (PubMed=39079530).
Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:7059; MGMT (Note=With p.Gly180_Asn207del and 19 mutations = SNAP-tag).
Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:13805; NEUROG2.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P33967; B.cereus Bsr.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; Q9QNF7; Human herpesvirus 1 TK.
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Ankle, skin; UBERON=UBERON_0001512.
Cell type: Fibroblast of skin; CL=CL_0002620.
Disease Multiple system atrophy (NCIt: C84909)
Multiple system atrophy (ORDO: Orphanet_102)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_E3CP (MSA-5)
Sex of cell Female
Age at sampling 60Y
Category Induced pluripotent stem cell
Publications

PubMed=39079530; DOI=10.1016/j.neuron.2024.06.002; PMCID=PMC11377155
Lam I., Ndayisaba A., Lewis A.J., Fu Y.-H., Sagredo G.T., Kuzkina A., Zaccagnini L., Celikag M., Sandoe J., Sanz R.L., Vahdatshoar A., Martin T.D., Morshed N., Ichihashi T., Tripathi A., Ramalingam N., Oettgen-Suazo C., Bartels T., Boussouf M., Schabinger M., Hallacli E., Jiang X., Verma A., Tea C., Wang Z.-C., Hakozaki H., Yu X., Hyles K., Park C., Wang X.-Y., Theunissen T.W., Wang H.-Y., Jaenisch R., Lindquist S.L., Stevens B., Stefanova N., Wenning G.K., van de Berg W.D.J., Luk K.C., Sanchez-Pernaute R., Gomez-Esteban J.C., Felsky D., Kiyota Y., Sahni N., Yi S.S., Chung C.-Y., Stahlberg H., Ferrer I., Schoneberg J., Elledge S.J., Dettmer U., Halliday G.M., Bartels T., Khurana V.
Rapid iPSC inclusionopathy models shed light on formation, consequence, and molecular subtype of alpha-synuclein inclusions.
Neuron 112:2886-2909.e16(2024)

Entry history
Entry creation10-Sep-2024
Last entry update10-Apr-2025
Version number3