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Cellosaurus COS/Fucci (CVCL_E071)

[Text version]
Cell line name COS/Fucci
Synonyms COS-Fucci
Accession CVCL_E071
Resource Identification Initiative To cite this cell line use: COS/Fucci (RRID:CVCL_E071)
Comments Group: Non-human primate cell line.
Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci (Fluorescent Ubiquitination-based Cell Cycle Indicator) construct.
Characteristics: Transfected with the Fucci probe which consist of the fusion of monomeric Kusabira-Orange2 (mKO2) to the ubiquitination domain of human CDT1 (AA 30-120) and monomeric AzamiGreen (mAG) to the ubiquitination domain of human geminin (AA 1-110).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; HC91W; AzamiGreen (Note=Green fluorescent protein from G.fascicularis).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; 134NN; mKusabira-Orange2 (Note=mKO2, orange fluorescent protein derived from F.concinna Kusabira-Orange (KO)).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:24576; CDT1 (Note=With p.Met1_Thr29del and p.Ser121_Leu546del).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:17493; GMNN (Note=With p.Tyr111_Ile209del).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pSV6-1).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Species of origin Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops) (NCBI Taxonomy: 9534)
Hierarchy Parent: CVCL_0224 (COS-7)
Sex of cell Male
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=18267078; DOI=10.1016/j.cell.2007.12.033
Asako Sakaue-Sawano, Hiroshi Kurokawa, Toshifumi Morimura, Aki Hanyu, Hiroshi Hama, Hatsuki Osawa, Saori Kashiwagi, Kiyoko Fukami, Takaki Miyata, Hiroyuki Miyoshi, Takeshi Imamura ...Show all 14 authors... , Masaharu Ogawa, Hisao Masai, Atsushi Miyawaki; Show fewer authors
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Cell 132:487-498(2008)

Cross-references
Cell line collections (Providers) RCB; RCB2814
Cell line databases/resources CLO; CLO_0050516
Encyclopedic resources Wikidata; Q54814317
Entry history
Entry creation22-Oct-2012
Last entry update10-Apr-2025
Version number17