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Cellosaurus HeLa/Fucci (CVCL_E070)

[Text version]
Cell line name HeLa/Fucci
Synonyms HeLa/Fucci #4; HeLa.S-Fucci
Accession CVCL_E070
Resource Identification Initiative To cite this cell line use: HeLa/Fucci (RRID:CVCL_E070)
Comments Population: African American.
Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci (Fluorescent Ubiquitination-based Cell Cycle Indicator) construct.
Characteristics: Transfected with the Fucci probe which consist of the fusion of monomeric Kusabira-Orange 2 (mKO2) to the ubiquitination domain of human CDT1 (aa 30-120) and monomeric Azami-Green (mAG) to the ubiquitination domain of human geminin (aa 1-110).
Transfected with: HGNC; 24576; CDT1 (with p.Met1_Thr29del and p.Ser121_Leu546del).
Transfected with: HGNC; 17493; GMNN (with p.Tyr111_Ile209del).
Transfected with: UniProtKB; Q60I24; Galaxea fascicularis monomeric Azami-Green.
Transfected with: UniProtKB; Q6I7B2; Fungia concinna monomeric Kusabira-Orange (with p.Lys49Glu, p.Pro70Val, p.Phe176Met, p.Lys185Glu, P.Lys188Glu, p.Ser192Asp, p.Ser196Gly and p.Leu210Gln = mKO2).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line

PubMed=18267078; DOI=10.1016/j.cell.2007.12.033
Sakaue-Sawano A., Kurokawa H., Morimura T., Hanyu A., Hama H., Osawa H., Kashiwagi S., Fukami K., Miyata T., Miyoshi H., Imamura T., Ogawa M., Masai H., Miyawaki A.
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Cell 132:487-498(2008)

Cell line collections (Providers) RCB; RCB2812
Cell line databases/resources CLO; CLO_0050931
Encyclopedic resources Wikidata; Q54882691
Entry history
Entry creation22-Oct-2012
Last entry update05-Oct-2023
Version number14