ID   HeLa/Fucci
AC   CVCL_E070
SY   HeLa/Fucci #4; HeLa.S-Fucci
DR   CLO; CLO_0050931
DR   RCB; RCB2812
DR   Wikidata; Q54882691
RX   PubMed=18267078;
CC   Population: African American.
CC   Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci (Fluorescent Ubiquitination-based Cell Cycle Indicator) construct.
CC   Characteristics: Transfected with the Fucci probe which consist of the fusion of monomeric Kusabira-Orange2 (mKO2) to the ubiquitination domain of human CDT1 (aa 30-120) and monomeric AzamiGreen (mAG) to the ubiquitination domain of human geminin (aa 1-110).
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; HC91W; AzamiGreen (Note=Green fluorescent protein from G.fascicularis).
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; 134NN; mKusabira-Orange2 (Note=mKO2, orange fluorescent protein derived from F.concinna Kusabira-Orange (KO)).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:24576; CDT1 (Note=With p.Met1_Thr29del and p.Ser121_Leu546del).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:17493; GMNN (Note=With p.Tyr111_Ile209del).
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC   Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
DI   NCIt; C27677; Human papillomavirus-related endocervical adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0030 ! HeLa
SX   Female
AG   30Y6M
CA   Cancer cell line
DT   Created: 22-10-12; Last updated: 10-04-25; Version: 17
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RX   PubMed=18267078; DOI=10.1016/j.cell.2007.12.033;
RA   Sakaue-Sawano A., Kurokawa H., Morimura T., Hanyu A., Hama H.,
RA   Osawa H., Kashiwagi S., Fukami K., Miyata T., Miyoshi H., Imamura T.,
RA   Ogawa M., Masai H., Miyawaki A.;
RT   "Visualizing spatiotemporal dynamics of multicellular cell-cycle
RT   progression.";
RL   Cell 132:487-498(2008).
//