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Cellosaurus U2OS dCas9-VPR (CVCL_DX84)

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Cell line name U2OS dCas9-VPR
Accession CVCL_DX84
Resource Identification Initiative To cite this cell line use: U2OS dCas9-VPR (RRID:CVCL_DX84)
Comments Population: Caucasian.
Characteristics: Transduced with a lentiviral vector EF1a-Bsd-T2A-dCas9-VPR-WPRE, where VPR is a tripartite activation system made of a fusion of VP64, p65(relA), and RTA (Horizon_Discovery=HD+dCas9-VPR-012).
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala = dCas9).
Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
Transfected with: MGI:103290; Rela (with p.Met1_Leu368 del).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Cell line collections (Providers) Horizon_Discovery; HD+dCas9-VPR-012
Entry history
Entry creation30-Jan-2024
Last entry update30-Jan-2024
Version number1