ID   U2OS dCas9-VPR
AC   CVCL_DX84
DR   Horizon_Discovery; HD+dCas9-VPR-012
CC   Population: Caucasian.
CC   Characteristics: Transduced with a lentiviral vector EF1a-Bsd-T2A-dCas9-VPR-WPRE, where VPR is a tripartite activation system made of a fusion of VP64, p65(relA), and RTA (Horizon_Discovery=HD+dCas9-VPR-012).
CC   Sequence variation: Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala = dCas9).
CC   Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
CC   Transfected with: MGI:103290; Rela (with p.Met1_Leu368 del).
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0042 ! U2OS
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 30-01-24; Last updated: 30-01-24; Version: 1
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