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Cellosaurus PFV (CVCL_DR64)

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Cell line name PFV
Synonyms Pig Flip-in Visualize
Accession CVCL_DR64
Resource Identification Initiative To cite this cell line use: PFV (RRID:CVCL_DR64)
Comments Characteristics: Transfected with a construct that contains a Sleeping Beauty (SB) DNA transposon, a puromycin resistance gene, an EGFP reporter gene and an Flp recombinase site for locus-directed gene insertion.
Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Skin; UBERON=UBERON_0002097.
Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin Sus scrofa (Pig) (NCBI Taxonomy: 9823)
Breed/subspecies: Gottingen minipig; VBO=VBO_0016899.
Sex of cell Male
Age at sampling <1M
Category Spontaneously immortalized cell line
Publications

PubMed=20336379; DOI=10.1007/s11033-010-0089-z
Jannik E. Jakobsen, Juan Li, Brian Moldt, Peter M. Kragh, Henrik Callesen, Jens Michael Hertz, Lars Bolund, Arne Lund Jorgensen, Jacob Giehm Mikkelsen, Anders Lade Nielsen;
Establishment of a pig fibroblast-derived cell line for locus-directed transgene expression in cell cultures and blastocysts.
Mol. Biol. Rep. 38:151-161(2011)

Cross-references
Encyclopedic resources Wikidata; Q54947303
Entry history
Entry creation13-Jul-2016
Last entry update14-Aug-2025
Version number10