ID   PFV
AC   CVCL_DR64
SY   Pig Flip-in Visualize
DR   Wikidata; Q54947303
RX   PubMed=20336379;
CC   Characteristics: Transfected with a construct that contains a Sleeping Beauty (SB) DNA transposon, a puromycin resistance gene, an EGFP reporter gene and an Flp recombinase site for locus-directed gene insertion.
CC   Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Derived from site: In situ; Skin; UBERON=UBERON_0002097.
CC   Cell type: Fibroblast of skin; CL=CL_0002620.
CC   Breed/subspecies: Gottingen minipig; VBO=VBO_0016899.
OX   NCBI_TaxID=9823; ! Sus scrofa (Pig)
SX   Male
AG   <1M
CA   Spontaneously immortalized cell line
DT   Created: 13-07-16; Last updated: 14-08-25; Version: 10
//
RX   PubMed=20336379; DOI=10.1007/s11033-010-0089-z;
RA   Jakobsen, Jannik E.
RA   Li, Juan
RA   Moldt, Brian
RA   Kragh, Peter M.
RA   Callesen, Henrik
RA   Hertz, Jens Michael
RA   Bolund, Lars
RA   Jorgensen, Arne Lund
RA   Mikkelsen, Jacob Giehm
RA   Nielsen, Anders Lade
RT   "Establishment of a pig fibroblast-derived cell line for locus-directed
RT   transgene expression in cell cultures and blastocysts.";
RL   Mol. Biol. Rep. 38:151-161(2011).
//