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Cellosaurus L-WRN (CVCL_DA06)

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Cell line name L-WRN
Synonyms L-Wnt3a/R-spondin/Noggin
Accession CVCL_DA06
Resource Identification Initiative To cite this cell line use: L-WRN (RRID:CVCL_DA06)
Comments Genetic integration: Method=Transfection; Gene=MGI; MGI:104327; Nog.
Genetic integration: Method=Transfection; Gene=MGI; MGI:1920030; Rspo3.
Genetic integration: Method=Transfection; Gene=MGI; MGI:98956; Wnt3a.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: In situ; Connective tissue; UBERON=UBERON_0002384.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C3H/An.
Hierarchy Parent: CVCL_0635 (L Wnt-3A)
Sex of cell Male
Age at sampling 100D
Category Spontaneously immortalized cell line
STR profile Source(s): CLS=300641

Markers:
Mouse STR 1-110
Mouse STR 1-217,18
Mouse STR 2-19
Mouse STR 3-214
Mouse STR 4-220.3
Mouse STR 5-514
Mouse STR 6-417,19
Mouse STR 6-712
Mouse STR 7-125,26
Mouse STR 8-116
Mouse STR 11-215,16
Mouse STR 12-116
Mouse STR 13-117
Mouse STR 15-324.3,25.3
Mouse STR 17-214,15
Mouse STR 18-315,16
Mouse STR 19-212
Mouse STR X-126

Run an STR similarity search on this cell line
Publications

PubMed=24232249; DOI=10.1038/nprot.2013.153; PMCID=PMC3969856
Hiroyuki Miyoshi, Thaddeus S. Stappenbeck;
In vitro expansion and genetic modification of gastrointestinal stem cells in spheroid culture.
Nat. Protoc. 8:2471-2482(2013)

Cross-references
Cell line collections (Providers) AddexBio; S0011002/4939
ATCC; CRL-3276
CLS; 300641
Encyclopedic resources Wikidata; Q54900914
Entry history
Entry creation13-Jul-2016
Last entry update14-Aug-2025
Version number12