ID   L-WRN
AC   CVCL_DA06
SY   L-Wnt3a/R-spondin/Noggin
DR   AddexBio; S0011002/4939
DR   ATCC; CRL-3276
DR   CLS; 300641
DR   Wikidata; Q54900914
RX   PubMed=24232249;
CC   Genetic integration: Method=Transfection; Gene=MGI; MGI:104327; Nog.
CC   Genetic integration: Method=Transfection; Gene=MGI; MGI:1920030; Rspo3.
CC   Genetic integration: Method=Transfection; Gene=MGI; MGI:98956; Wnt3a.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Connective tissue; UBERON=UBERON_0002384.
CC   Cell type: Fibroblast; CL=CL_0000057.
CC   Breed/subspecies: C3H/An.
ST   Source(s): CLS=300641
ST   Mouse STR 1-1: 10
ST   Mouse STR 1-2: 17,18
ST   Mouse STR 2-1: 9
ST   Mouse STR 3-2: 14
ST   Mouse STR 4-2: 20.3
ST   Mouse STR 5-5: 14
ST   Mouse STR 6-4: 17,19
ST   Mouse STR 6-7: 12
ST   Mouse STR 7-1: 25,26
ST   Mouse STR 8-1: 16
ST   Mouse STR 11-2: 15,16
ST   Mouse STR 12-1: 16
ST   Mouse STR 13-1: 17
ST   Mouse STR 15-3: 24.3,25.3
ST   Mouse STR 17-2: 14,15
ST   Mouse STR 18-3: 15,16
ST   Mouse STR 19-2: 12
ST   Mouse STR X-1: 26
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0635 ! L Wnt-3A
SX   Male
AG   100D
CA   Spontaneously immortalized cell line
DT   Created: 13-07-16; Last updated: 10-04-25; Version: 11
//
RX   PubMed=24232249; DOI=10.1038/nprot.2013.153; PMCID=PMC3969856;
RA   Miyoshi H., Stappenbeck T.S.;
RT   "In vitro expansion and genetic modification of gastrointestinal stem
RT   cells in spheroid culture.";
RL   Nat. Protoc. 8:2471-2482(2013).
//