Cellosaurus 4T1-Fluc-Neo (monoclonal) (CVCL_D5LJ)
| Cell line name | 4T1-Fluc-Neo (monoclonal) |
|---|---|
| Accession | CVCL_D5LJ |
| Resource Identification Initiative | To cite this cell line use: 4T1-Fluc-Neo (monoclonal) (RRID:CVCL_D5LJ) |
| Comments | Characteristics: Reporter cell line, transduced with the LV-Fluc-P2A-Neo vector encoding for luciferase linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter (from parent cell line). Characteristics: A monoclonal population was isolated using three rounds of selection in a methylcellulose-based semi-solid medium (Imanis=CL126). Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG). Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo. Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase. Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911. |
| Disease | Malignant neoplasms of the mouse mammary gland (NCIt: C21678) |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: BALB/cfC3H. |
| Hierarchy | Parent: CVCL_QZ57 (4T1-Fluc-Neo) |
| Sex of cell | Female |
| Age at sampling | Age unspecified |
| Category | Cancer cell line |
| Publications | PubMed=30674994; DOI=10.1038/s41417-019-0081-2; PMCID=PMC7170803 |
| Cross-references | |
| Cell line collections (Providers) | Imanis; CL126 |
| Encyclopedic resources | Wikidata; Q127378748 |
| Entry history | |
| Entry creation | 02-May-2024 |
| Last entry update | 14-Aug-2025 |
| Version number | 4 |