Cellosaurus cell line MDCK-4KO-EKARrEV-NLS (CVCL

Cellosaurus MDCK-4KO-EKARrEV-NLS (CVCL_D5IH)

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Cell line name

MDCK-4KO-EKARrEV-NLS

Accession

CVCL_D5IH

Resource Identification Initiative

To cite this cell line use: MDCK-4KO-EKARrEV-NLS (RRID:CVCL_D5IH)

Comments

Characteristics: Transduced with the FRET biosensor EKARrEV-NLS FRET biosensor EKARrEV-NLS, which consists of a donor fluorescent protein CFP, an ERK substrate peptide derived from RSK1, an optimized linker, a FHA1 phosphate binding domain, an acceptor fluorescent protein YFP and a nuclear localization signal (PubMed=34667080).
Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:40235; EGF.
Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:111490; EREG.
Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:41609; HBEGF.
Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:112755; TGFA.
Genetic integration: Method=Transduction; Gene=FPbase; ZRLU4; CFP (Note=Cyan fluorescent protein derived from GFP).
Genetic integration: Method=Transduction; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Cell type: Epithelial cell of kidney; CL=CL_0002518.

Species of origin

Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372.

Hierarchy

Parent: CVCL_D5I5 (MDCK-dEGF/HBEGF/TGFalpha/EREG KO 1)
Children:

CVCL_D5II (MDCK-4KO-EKARrEV-NLS-EGF)	CVCL_D5IJ (MDCK-4KO-EKARrEV-NLS-EREG)	CVCL_D5IK (MDCK-4KO-EKARrEV-NLS-HBEGF)
CVCL_D5IM (MDCK-4KO-EKARrEV-NLS-loxP-NRG1)	CVCL_D5IL (MDCK-4KO-EKARrEV-NLS-TGFalpha)

Sex of cell

Female

Age at sampling

Adult