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Cellosaurus BY-2 (CVCL_D5GM)

[Text version]
Cell line name BY-2
Synonyms BY2; TBY-2; TBY2; Tobacco BY-2; NT-1; NT1; Nicotiana tabacum-1
Accession CVCL_D5GM
Resource Identification Initiative To cite this cell line use: BY-2 (RRID:CVCL_D5GM)
Comments Group: Plant cell line.
Characteristics: Rapid cell proliferation (PubMed=15638240).
Characteristics: Can be used to prepare protoplasts (PubMed=17947977).
Characteristics: Produces low levels of nicotine (PubMed=18567891).
Characteristics: Tested for the production of animal proteins (PubMed=7766897).
Characteristics: Tested for the production of bacterial proteins (PubMed=14688094; PubMed=22928883).
Characteristics: Tested for the production of synthetic proteins (PubMed=15818563).
Characteristics: Tested for the production of viral proteins (PubMed=14680934; PubMed=12601177; PubMed=16187337).
Characteristics: Used as model for the electrochemical sensing of beta-glucuronidase activity of transgenic cell lines (PubMed=29982119).
Doubling time: 15.8 hours (DOI=10.1016/S0074-7696(08)62452-3).
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Anecdotal: BY-2 cell line was generated at the Japan Tobacco and Salt Monopoly Public Corporation. In 1982 Toshiyuki Nagata brought it with him to the USA and left it there after renaming it to NT-1 in order to distribute it clandestinely because he was not allowed officially to give the BY-2 cells to others. He called it NT-1 as it could both mean Nicotiana tabacum or Nagata, Toshiyuki (PubMed=37642778).
Derived from site: In situ; Seedling.
Species of origin Nicotiana tabacum (Common tobacco) (NCBI Taxonomy: 4097)
Breed/subspecies: Cultivar Bright Yellow 2.
Hierarchy Children:
CVCL_D5GN (BY-GF11)CVCL_D5GP (BY-GV7)CVCL_D5GR (TBY2-41/ST)
CVCL_D5GS (TBY2-AtRER1B)CVCL_D5GT (TBY2-R31)
Category Spontaneously immortalized cell line
Web pages https://en.wikipedia.org/wiki/Tobacco_BY-2_cells
Publications

CLPUB00765
Kato K., Matsumoto T., Koiwai A., Mizusaki S., Nishida K., Noguchi M., Tamaki E.
Liquid suspension culture of tobacco cells.
(In) Fermentation technology today. Proceedings of the IVth international fermentation symposium; Terui G. (eds.); pp.689-695; Soc. Ferment. Technol.; Osaka (1972)

PubMed=16664453; DOI=10.1104/pp.79.2.568
An G.
High efficiency transformation of cultured tobacco cells.
Plant Physiol. 79:568-570(1985)

DOI=10.1016/S0074-7696(08)62452-3
Nagata T., Nemoto Y., Hasezawa S.
Tobacco BY-2 cell line as the HeLa cell in the cell biology of higher plants.
Int. Rev. Cytol. 132:1-30(1992)

PubMed=7766897; DOI=10.1007/BF00020889
Matsumoto S., Ikura K., Ueda M., Sasaki R.
Characterization of a human glycoprotein (erythropoietin) produced in cultured tobacco cells.
Plant Mol. Biol. 27:1163-1172(1995)

PubMed=12601177; DOI=10.1073/pnas.0438037100
Sojikul P., Buehner N., Mason H.S.
A plant signal peptide-hepatitis B surface antigen fusion protein with enhanced stability and immunogenicity expressed in plant cells.
Proc. Natl. Acad. Sci. U.S.A. 100:2209-2214(2003)

PubMed=14680934; DOI=10.1016/j.pep.2003.07.004
Sunil Kumar G.B., Ganapathi T.R., Revathi C.J., Prasad K.S., Bapat V.A.
Expression of hepatitis B surface antigen in tobacco cell suspension cultures.
Protein Expr. Purif. 32:10-17(2003)

PubMed=14688094; DOI=10.1128/IAI.72.1.168-175.2004
Judge N.A., Mason H.S., O'Brien A.D.
Plant cell-based intimin vaccine given orally to mice primed with intimin reduces time of Escherichia coli O157:H7 shedding in feces.
Infect. Immun. 72:168-175(2004)

PubMed=15638240; DOI=10.1007/s11745-004-1289-0
Hemmerlin A., Gerber E., Feldtrauer J.-F., Wentzinger L., Hartmann M.-A., Tritsch D., Hoeffler J.-F., Rohmer M., Bach T.J.
A review of tobacco BY-2 cells as an excellent system to study the synthesis and function of sterols and other isoprenoids.
Lipids 39:723-735(2004)

PubMed=15818563; DOI=10.1002/bit.20441
Xu J.-F., Shpak E., Gu T.-Y., Moo-Young M., Kieliszewski M.
Production of recombinant plant gum with tobacco cell culture in bioreactor and gum characterization.
Biotechnol. Bioeng. 90:578-588(2005)

DOI=10.1007/3-540-32674-X_8
Petrasek J., Zazimalova E.
The BY-2 cell line as a tool to study auxin transport.
Biotechnol. Agric. For. 58:107-117(2006)

PubMed=16187337; DOI=10.1002/bit.20695
Zhang X.-R., Mason H.S.
Bean yellow dwarf virus replicons for high-level transgene expression in transgenic plants and cell cultures.
Biotechnol. Bioeng. 93:271-279(2006)

PubMed=17406390; DOI=10.1038/nprot.2006.176
Mayo K.J., Gonzales B.J., Mason H.S.
Genetic transformation of tobacco NT1 cells with Agrobacterium tumefaciens.
Nat. Protoc. 1:1105-1111(2006)

PubMed=17406517; DOI=10.1038/nprot.2006.381
Kumagai-Sano F., Hayashi T., Sano T., Hasezawa S.
Cell cycle synchronization of tobacco BY-2 cells.
Nat. Protoc. 1:2621-2627(2006)

PubMed=17947977; DOI=10.1038/nprot.2007.360
Miao Y.-S., Jiang L.-W.
Transient expression of fluorescent fusion proteins in protoplasts of suspension cultured cells.
Nat. Protoc. 2:2348-2353(2007)

PubMed=18567891; DOI=10.1093/pcp/pcn096
Shoji T., Hashimoto T.
Why does anatabine, but not nicotine, accumulate in jasmonate-elicited cultured tobacco BY-2 cells?
Plant Cell Physiol. 49:1209-1216(2008)

DOI=10.4067/S0718-07642010000200002
Ruiz L.F., Higareda A.E., Pardo M.A.
Synchronization of tobacco cells (Nicotiana tabacum) NT-1.
Inf. Tecnol. 21:3-11(2010)

PubMed=22055919; DOI=10.1016/j.jbiosc.2011.09.022
Ullisch D.A., Muller C.A., Maibaum S., Kirchhoff J., Schiermeyer A., Schillberg S., Roberts J.L., Treffenfeldt W., Buchs J.
Comprehensive characterization of two different Nicotiana tabacum cell lines leads to doubled GFP and HA protein production by media optimization.
J. Biosci. Bioeng. 113:242-248(2012)

PubMed=22928883; DOI=10.1080/03079457.2012.709606
Miller T., Fanton M., Nickelson S., Mason H.S., Webb S.
Safety and immunogenicity of bacterial and tobacco plant cell line derived recombinant native and mutant Escherichia coli heat-labile toxin in chickens.
Avian Pathol. 41:441-449(2012)

PubMed=29982119; DOI=10.1016/j.bios.2018.06.045
Pandey R., Teig-Sussholz O., Schuster S., Avni A., Shacham-Diamand Y.
Integrated electrochemical Chip-on-Plant functional sensor for monitoring gene expression under stress.
Biosens. Bioelectron. 117:493-500(2018)

PubMed=37642778; DOI=10.1007/s10265-023-01490-4
Nagata T.
Hidden history of the tobacco BY-2 cell line.
J. Plant Res. 136:781-786(2023)

Cross-references
Cell line collections (Providers) RIKEN_BRC_EPD; rpc00001
Gene expression databases GEO; GSE6948
GEO; GSE6949
GEO; GSE14250
GEO; GSE37649
GEO; GSE42562
GEO; GSE111980
GEO; GSE160438
GEO; GSE193131
Entry history
Entry creation02-May-2024
Last entry update02-May-2024
Version number1