ID   BY-2
AC   CVCL_D5GM
SY   BY2; TBY-2; TBY2; Tobacco BY-2; NT-1; NT1; Nicotiana tabacum-1
DR   BTO; BTO_0001972
DR   EFO; EFO_0022754
DR   DSMZ; PC-1181
DR   GEO; GSE6948
DR   GEO; GSE6949
DR   GEO; GSE14250
DR   GEO; GSE37649
DR   GEO; GSE42562
DR   GEO; GSE111980
DR   GEO; GSE160438
DR   GEO; GSE193131
DR   RIKEN_BRC_EPD; rpc00001
DR   Wikidata; Q7811455
RX   CelloPub=CLPUB00765;
RX   DOI=10.1007/3-540-32674-X_8;
RX   DOI=10.1016/S0074-7696(08)62452-3;
RX   DOI=10.4067/S0718-07642010000200002;
RX   PubMed=7766897;
RX   PubMed=10728644;
RX   PubMed=12601177;
RX   PubMed=14680934;
RX   PubMed=14688094;
RX   PubMed=15638240;
RX   PubMed=15818563;
RX   PubMed=16187337;
RX   PubMed=16664453;
RX   PubMed=17406390;
RX   PubMed=17406517;
RX   PubMed=17947977;
RX   PubMed=18567891;
RX   PubMed=22055919;
RX   PubMed=22928883;
RX   PubMed=29982119;
RX   PubMed=37642778;
WW   Info; Wikipedia; -; https://en.wikipedia.org/wiki/Tobacco_BY-2_cells
CC   Group: Plant cell line.
CC   Characteristics: Can be used to prepare protoplasts (PubMed=17947977).
CC   Characteristics: Produces low levels of nicotine (PubMed=18567891).
CC   Characteristics: Tested for the production of animal proteins (PubMed=7766897).
CC   Characteristics: Tested for the production of bacterial proteins (PubMed=14688094; PubMed=22928883).
CC   Characteristics: Tested for the production of synthetic proteins (PubMed=15818563).
CC   Characteristics: Tested for the production of viral proteins (PubMed=14680934; PubMed=12601177; PubMed=16187337).
CC   Characteristics: Used as model for the electrochemical sensing of beta-glucuronidase activity of transgenic cell lines (PubMed=29982119).
CC   Doubling time: 15.8 hours (DOI=10.1016/S0074-7696(08)62452-3).
CC   Omics: Transcriptomics; Microarray.
CC   Omics: Transcriptomics; RNAseq.
CC   Anecdotal: This cell line was generated at the Japan Tobacco and Salt Monopoly Public Corporation. In 1982 Toshiyuki Nagata brought it with him to the USA and left it there after renaming it to NT-1 in order to distribute it clandestinely because he was not allowed officially to give the BY-2 cell line to others. He called it NT-1 as it could both mean Nicotiana tabacum or Nagata, Toshiyuki (PubMed=37642778).
CC   Discontinued: DSMZ; PC-1181; true.
CC   Derived from site: In situ; Seedling; PO=PO_0008037.
CC   Breed/subspecies: Cultivar Bright Yellow 2.
OX   NCBI_TaxID=4097; ! Nicotiana tabacum (Common tobacco)
CA   Spontaneously immortalized cell line
DT   Created: 02-05-24; Last updated: 27-11-25; Version: 5
CH   CVCL_D5GN ! BY-GF11
CH   CVCL_D5GP ! BY-GV7
CH   CVCL_D5GR ! TBY2-41/ST
CH   CVCL_D5GS ! TBY2-AtRER1B
CH   CVCL_D5GT ! TBY2-R31
//
RX   PubMed=17947977; DOI=10.1038/nprot.2007.360;
RA   Miao, Yan-Song
RA   Jiang, Li-Wen
RT   "Transient expression of fluorescent fusion proteins in protoplasts of
RT   suspension cultured cells.";
RL   Nat. Protoc. 2:2348-2353(2007).
//
RX   PubMed=18567891; DOI=10.1093/pcp/pcn096;
RA   Shoji, Tsubasa
RA   Hashimoto, Takashi
RT   "Why does anatabine, but not nicotine, accumulate in
RT   jasmonate-elicited cultured tobacco BY-2 cells?";
RL   Plant Cell Physiol. 49:1209-1216(2008).
//
RX   PubMed=15638240; DOI=10.1007/s11745-004-1289-0;
RA   Hemmerlin, Andrea
RA   Gerber, Esther
RA   Feldtrauer, Jean-Francois
RA   Wentzinger, Laurent
RA   Hartmann, Marie-Andree
RA   Tritsch, Denis
RA   Hoeffler, Jean-Francois
RA   Rohmer, Michel
RA   Bach, Thomas J.
RT   "A review of tobacco BY-2 cells as an excellent system to study the
RT   synthesis and function of sterols and other isoprenoids.";
RL   Lipids 39:723-735(2004).
//
RX   DOI=10.1007/3-540-32674-X_8;
RA   Petrasek, Jan
RA   Zazimalova, Eva
RT   "The BY-2 cell line as a tool to study auxin transport.";
RL   Biotechnol. Agric. For. 58:107-117(2006).
//
RX   PubMed=22055919; DOI=10.1016/j.jbiosc.2011.09.022;
RA   Ullisch, David Alexander
RA   Muller, Christina A.
RA   Maibaum, Sabrina
RA   Kirchhoff, Janina
RA   Schiermeyer, Andreas
RA   Schillberg, Stefan
RA   Roberts, Jean L.
RA   Treffenfeldt, Wiltrud
RA   Buchs, Jochen
RT   "Comprehensive characterization of two different Nicotiana tabacum
RT   cell lines leads to doubled GFP and HA protein production by media
RT   optimization.";
RL   J. Biosci. Bioeng. 113:242-248(2012).
//
RX   DOI=10.1016/S0074-7696(08)62452-3;
RA   Nagata, Toshiyuki
RA   Nemoto, Yasuyuki
RA   Hasezawa, Seiichiro
RT   "Tobacco BY-2 cell line as the HeLa cell in the cell biology of higher
RT   plants.";
RL   Int. Rev. Cytol. 132:1-30(1992).
//
RX   DOI=10.4067/S0718-07642010000200002;
RA   Ruiz, Leon F.
RA   Higareda, Ana E.
RA   Pardo, Marco A.
RT   "Synchronization of tobacco cells (Nicotiana tabacum) NT-1.";
RL   Inf. Tecnol. 21:3-11(2010).
//
RX   CelloPub=CLPUB00765;
RA   Kato, Kumio
RA   Matsumoto, Takashi
RA   Koiwai, Akira
RA   Mizusaki, Shigenobu
RA   Nishida, Koh
RA   Noguchi, Masao
RA   Tamaki, Einosuke
RT   "Liquid suspension culture of tobacco cells.";
RL   (In book chapter) Fermentation technology today. Proceedings of the IVth international fermentation symposium; Terui, Gyozo (eds.); pp.689-695; Society of Fermentation Technology; Osaka; Japan (1972).
//
RX   PubMed=17406517; DOI=10.1038/nprot.2006.381;
RA   Kumagai-Sano, Fumi
RA   Hayashi, Tomomi
RA   Sano, Toshio
RA   Hasezawa, Seiichiro
RT   "Cell cycle synchronization of tobacco BY-2 cells.";
RL   Nat. Protoc. 1:2621-2627(2006).
//
RX   PubMed=16664453; DOI=10.1104/pp.79.2.568; PMCID=PMC1074928;
RA   An, Gynheung
RT   "High efficiency transformation of cultured tobacco cells.";
RL   Plant Physiol. 79:568-570(1985).
//
RX   PubMed=29982119; DOI=10.1016/j.bios.2018.06.045;
RA   Pandey, Richa
RA   Teig-Sussholz, Orian
RA   Schuster, Silvia
RA   Avni, Adi
RA   Shacham-Diamand, Yosi
RT   "Integrated electrochemical Chip-on-Plant functional sensor for
RT   monitoring gene expression under stress.";
RL   Biosens. Bioelectron. 117:493-500(2018).
//
RX   PubMed=10728644; DOI=10.1023/A:1009832822096;
RA   Nagata, Toshiyuki
RA   Kumagai, Fumi
RT   "Plant cell biology through the window of the highly synchronized
RT   tobacco BY-2 cell line.";
RL   Methods Cell Sci. 21:123-127(1999).
//
RX   PubMed=22928883; DOI=10.1080/03079457.2012.709606;
RA   Miller, Tim
RA   Fanton, Matthew
RA   Nickelson, Stephanie
RA   Mason, Hugh S.
RA   Webb, Steven
RT   "Safety and immunogenicity of bacterial and tobacco plant cell line
RT   derived recombinant native and mutant Escherichia coli heat-labile
RT   toxin in chickens.";
RL   Avian Pathol. 41:441-449(2012).
//
RX   PubMed=7766897; DOI=10.1007/BF00020889;
RA   Matsumoto, Shinya
RA   Ikura, Koji
RA   Ueda, Masatsugu
RA   Sasaki, Ryuzo
RT   "Characterization of a human glycoprotein (erythropoietin) produced in
RT   cultured tobacco cells.";
RL   Plant Mol. Biol. 27:1163-1172(1995).
//
RX   PubMed=12601177; DOI=10.1073/pnas.0438037100; PMCID=PMC151319;
RA   Sojikul, Punchapat
RA   Buehner, Norene
RA   Mason, Hugh S.
RT   "A plant signal peptide-hepatitis B surface antigen fusion protein
RT   with enhanced stability and immunogenicity expressed in plant
RT   cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:2209-2214(2003).
//
RX   PubMed=14688094; DOI=10.1128/IAI.72.1.168-175.2004; PMCID=PMC343997;
RA   Judge, Nicole A.
RA   Mason, Hugh S.
RA   O'Brien, Alison D.
RT   "Plant cell-based intimin vaccine given orally to mice primed with
RT   intimin reduces time of Escherichia coli O157:H7 shedding in feces.";
RL   Infect. Immun. 72:168-175(2004).
//
RX   PubMed=17406390; DOI=10.1038/nprot.2006.176;
RA   Mayo, Kristin J.
RA   Gonzales, Barbara J.
RA   Mason, Hugh S.
RT   "Genetic transformation of tobacco NT1 cells with Agrobacterium
RT   tumefaciens.";
RL   Nat. Protoc. 1:1105-1111(2006).
//
RX   PubMed=15818563; DOI=10.1002/bit.20441;
RA   Xu, Jian-Feng
RA   Shpak, Elena
RA   Gu, Ting-Yue
RA   Moo-Young, Murray
RA   Kieliszewski, Marcia J.
RT   "Production of recombinant plant gum with tobacco cell culture in
RT   bioreactor and gum characterization.";
RL   Biotechnol. Bioeng. 90:578-588(2005).
//
RX   PubMed=16187337; DOI=10.1002/bit.20695;
RA   Zhang, Xiu-Ren
RA   Mason, Hugh S.
RT   "Bean yellow dwarf virus replicons for high-level transgene expression
RT   in transgenic plants and cell cultures.";
RL   Biotechnol. Bioeng. 93:271-279(2006).
//
RX   PubMed=37642778; DOI=10.1007/s10265-023-01490-4; PMCID=PMC10587202;
RA   Nagata, Toshiyuki
RT   "Hidden history of the tobacco BY-2 cell line.";
RL   J. Plant Res. 136:781-786(2023).
//
RX   PubMed=14680934; DOI=10.1016/j.pep.2003.07.004;
RA   Sunil Kumar, Gujulla B.
RA   Ganapathi, Thumballi R.
RA   Revathi, Chaganti J.
RA   Prasad, K.S.N.
RA   Bapat, Vishwas A.
RT   "Expression of hepatitis B surface antigen in tobacco cell suspension
RT   cultures.";
RL   Protein Expr. Purif. 32:10-17(2003).
//