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Cellosaurus HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3 (CVCL_D4BX)

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Cell line name HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3
Synonyms Syn10 Galectin-3 FRET
Accession CVCL_D4BX
Resource Identification Initiative To cite this cell line use: HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3 (RRID:CVCL_D4BX)
Comments Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (from parent cell line).
Characteristics: Additional expression of two plasmids encoding mRuby3- and mClover3-tagged LGALS3, enabling a FRET-based readout of endolysosome damage (Direct_author_submission).
Transfected with: HGNC; 11138; SNCA (with p.Ala53Thr).
Transfected with: HGNC; 6563; LGALS3.
Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
Transfected with: UniProtKB; Q8ISF8; Entacmaea quadricolor eqFP611 (modified to produce the bright RFP mRuby3).
Transfected with: UniProtKB; P42212; GFP (with p.Ser30Arg, p.Tyr39Asn, p.Ser65Gly, p.Gln69Ala, p.Phe99Ser, p.Asn105Thr, p.Tyr145Phe, p.Asn149Tyr, p.Met153Thr, p.Gly160Cys, p.Val163Ala, p.Ile171Val, p.Thr203His, p.Ala206Lys = mClover3).
Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [Note=tsA].
Miscellaneous: Cell line information from personal communication of Yuste-Checa P.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_D4BW (HEK293T TetOff-SNCA-A53T)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Entry history
Entry creation30-Jan-2024
Last entry update30-Jan-2024
Version number1