Cellosaurus HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3 (CVCL_D4BX)
Cell line name | HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3 |
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Synonyms | Syn10 Galectin-3 FRET |
Accession | CVCL_D4BX |
Resource Identification Initiative | To cite this cell line use: HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3 (RRID:CVCL_D4BX) |
Comments | Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (from parent cell line). Characteristics: Additional expression of two plasmids encoding mRuby3- and mClover3-tagged LGALS3, enabling a FRET-based readout of endolysosome damage (Direct_author_submission). Transfected with: HGNC; 11138; SNCA (with p.Ala53Thr). Transfected with: HGNC; 6563; LGALS3. Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). Transfected with: UniProtKB; Q8ISF8; Entacmaea quadricolor eqFP611 (modified to produce the bright RFP mRuby3). Transfected with: UniProtKB; P42212; GFP (with p.Ser30Arg, p.Tyr39Asn, p.Ser65Gly, p.Gln69Ala, p.Phe99Ser, p.Asn105Thr, p.Tyr145Phe, p.Asn149Tyr, p.Met153Thr, p.Gly160Cys, p.Val163Ala, p.Ile171Val, p.Thr203His, p.Ala206Lys = mClover3). Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd). Transfected with: UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCBI_TaxID; 28285; Adenovirus 5. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [Note=tsA]. Miscellaneous: Cell line information from personal communication of Yuste-Checa P. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_D4BW (HEK293T TetOff-SNCA-A53T) |
Sex of cell | Female |
Age at sampling | Fetus |
Category | Transformed cell line |
Entry history | |
Entry creation | 30-Jan-2024 |
Last entry update | 30-Jan-2024 |
Version number | 1 |